I have a bldc driver with CAN Bus with CiA 402 protocol. STM32F446RE microcontroller is used to regulate the motive force by way of CAN bus. Stm32 Cube Ide is used to program the microcontroller.
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During regular improvement, the neural cell adhesion molecule N-CAM modifications on the cell-surface from a sialic acid-rich embryonic, or E kind, to a number of adult, or A varieties which have much less sialic acid (E-to-A conversion). To examine the mobile and molecular mechanisms that underlie these adjustments, we have established circumstances beneath which E-to-A conversion occurs in cultured explants of central nervous system tissues. Mouse cerebellum, chick spinal wire, and chick retina that express the E form of N-CAM were dissected and cultured on collagen gels. After 3-6 d in tradition, elevated proportions of A types have been synthesized, as revealed by particular immunoprecipitation and immunoblotting.
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The fee of E-to-A conversion and the proportions of the completely different A varieties synthesized in vitro had been similar to these noticed for the tissues in vivo at comparable times. These outcomes suggest that the post translational processing in vitro was similar to that in vivo. The data have been in preserving with the likelihood that the increase in synthesis of A types occurred both in cells that had beforehand synthesized E forms or in a distinct population of cells that already synthesized A types. In pulse-chase experiments, the E type of N-CAM that was synthesized through the first day after explantation persisted as E type for a quantity of days, at occasions when newly synthesized N-CAM was predominantly in A types.
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